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          關于我們首頁 > 產品中心 > 染料與染色劑 > invitrogen > 18064-022SuperScript® II Reverse Transcriptase
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          • SuperScript® II Reverse Transcriptase
          • 型號:18064-022
          • 報價:
          • 地區:上海市
          • 0
          • SuperScript® II Reverse Transcriptase 18064-022 *,價格超優!更多優惠!更多現貨,歡迎免費訂購: :、、
          • 025-58690567
          產品詳細介紹
          貨號

          單位規格

          目錄價格 (CNY)*
          18064-014
          10,000 units

          3,918.00

          詢價
          18064-022
          2,000 units

          1,142.00

          詢價

          18064-071
          4 x 10,000 units

          13,860.00

          詢價

          SuperScript? II Reverse Transcriptase 18064-022

          描述

          SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics.

          Yield – Reduced RNase H activity results in greater first-strand cDNA yields
          Size of cDNA – Generation of RT-PCR products up to 12 kb
          Thermostability – Full activity at 42°C
          Flexibility – Uses either total or poly(A)+ RNA

          Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.

          Unit Definition: One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).

          Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.

          For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

          SuperScript? II Reverse Transcriptase 18064-022

          規格

          Optimal Reaction Temperature:42° C
          Final Product Size(s):12.3 kb or less
          Reverse Transcriptase:SuperScript? II
          Ribonuclease H Activity:Reduced
          Form:Frozen
          Format:Tube(s)
          Quantity:2000 units
          Template:ssDNA, ssRNA, RNA-DNA Hybrid
          Sensitivity:Medium
          Product Size:2,000 units
          Concentration:200 U?μl
          Enzyme Function:RNA dependent DNA Polymerase
          Shipping Condition:Wet Ice
          Number of Reactions:10 Reactions

          SuperScript? II Reverse Transcriptase 18064-022

          內容及儲存

          SuperScript? II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
          Store at -20°C. Guaranteed stable for 6 months when properly stored.

          For Research Use Only. Not for use in diagnostic procedures.

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